^aPG & Research Department of Biotechnology, Nandha Arts and Science College, Erode – 638052, TamilNadu, India

^bSchool of Natural and Applied Sciences, Mulungushi University, Kabwe 80415,

Zambia

^cDepartment of Food Science and Nutrition, College of Food &

AgricultureSciences, King Saud University, Riyadh - 11451, Kingdom of Saudi

Arabia

^dDepartment of Oral Maxillofacial Surgery, Rajas Dental College and Hospital,

Kavalkinaru, Tirunelveli– 627105, Tamil Nadu, India

Copper nanoparticle synthesis and characterization are now being done widely due to its broad nanotechnology research interest, especially in medical applications. The current work set out to produce copper nanoparticles(CuNPs) by employing the herb Andrographis paniculata for medicinal purposes. Andrographis paniculata leaf extract was used to make CuNPs using copper sulphate (CuSO4). For monitoring the synthesis ofCuNPs, the UV-vis absorption spectra were obtained and Surface Plasmon resonance (SPR) peaks at 500nm.

The X-ray diffraction (XRD) pattern reveals the average size of the crystallites is 5.876 nm.

This environmentally friendly approach yields homogenous, spherical particles, as demonstrated by images of Scanning and Transmission electron microscopy (TEM and SEM). The outcomes demonstrated that leaf extract is most suited for producing CuNPs. It shows a greater zone of inhibition and inhibitory action against tested bacteria such as Bacillus cereus, Escherichia Coli, Klebsiella pneumoniae and Staphylococcus aureus, and it can be used for the inhibition of several harmful microbes. Because the synthesized CuNPs are non-toxic, environmentally benign, and suitable for usage in pharmaceutical and other fields, they can be applied in a variety of ways in future.